centrifuge polypropylene tubes  (Greiner Bio)

Journal: Current Protocols

Article Title: Isolation, Purification, and Comprehensive Flow Cytometry Assessment of Lung Stromal Cells

doi: 10.1002/cpz1.70078

Figure Lengend Snippet: Troubleshooting

Article Snippet: C57Bl/6J mice RPMI 1640 Medium (Thermo Fisher Scientific, cat. no. 61870036) Ice Digestion buffer (see recipe) Collection buffer (see recipe) ACK lysis buffer (Thermo Fisher Scientific, cat. no. A1049201) PBS, 1× (Thermo Fisher Scientific, cat. no. 10010023) Trypan blue stain, 0.4% (Thermo Fisher Scientific, cat. no. T10282) FBS (Thermo Fisher Scientific, cat. no. 10082147) EDTA, 0.5 M (Thermo Fisher Scientific, cat. no. AM9260G) HEPES, 1 M (Thermo Fisher Scientific, cat. no. 15630056) Collagenase P from Clostridium histolyticum (Merck, COLLP‐RO, cat. no. 11249002001) Collagenase II (Thermo Fisher Scientific, cat. no. 17101015) DNase I, grade II (Merck, cat. no. 10104159001) Eppendorf tubes: 15 mL (Sarstedt, cat. no. 72.706.400) 2 mL (Sarstedt, cat. no. 72.695.400) Bacterial Petri dishes, 10 cm (VWR, cat. no. 391‐2002) Fine‐tip scissors gentleMACS C Tubes (Miltenyi cat. no. 130‐093‐237) Water bath, 37°C gentleMACS Octo Dissociator with Heaters (Miltenyi, cat. no. 130‐096‐427) Polypropylene centrifuge tubes, 15 mL (Greiner Bio‐One GmbH, cat. no. 188271) Filter mesh, 100 μm (cut into 1.5 × 1.5–cm cubes and autoclaved; A. Hartenstein GmbH, cat. no. PES3) Centrifuge (Eppendorf, cat. no. 5810 R) Countess Cell Counting Chamber Slides (Thermo Fisher Scientific, cat. no. C10228) Countess II FL Automated Cell Counter (Thermo Fisher Scientific, cat. no. AMQAF1000)

Techniques: Activity Assay, Lysis, Suspension, Transferring, Purification, Isolation, Cell Counting

Journal: Current Protocols

Article Title: Isolation, Purification, and Comprehensive Flow Cytometry Assessment of Lung Stromal Cells

doi: 10.1002/cpz1.70078

Figure Lengend Snippet: Troubleshooting

Article Snippet: Lung cell suspension from Basic Protocol PBS, 1× (Thermo Fisher Scientific, cat. no. 10010023) eBioscience Fixable Viability Dye eFluor 780 (Thermo Fisher Scientific, cat. no. 65‐0865‐14) FC‐block mix (see recipe) Antibodies (see Table ) FACS buffer (see recipe) Fixation solution (see recipe) FBS (Thermo Fisher Scientific, cat. no. 10082147) EDTA, 0.5 M (Thermo Fisher Scientific, cat. no. AM9260G) Sodium azide solution, 20% (Morphisto, cat. no. 18189.00100) BSA solution, 10% w/w (Miltenyi, cat. no. 130‐091‐376) FcR blocking reagent (Miltenyi, cat. no.130‐092‐575) Paraformaldehyde in PBS, 4% (Morphisto, cat. no. 10303.00500) Eppendorf tubes, 1.5 mL (Sarstedt, cat. no 72.706.400) Polypropylene centrifuge tubes, 15 mL (Greiner Bio‐One GmbH, cat. no. 188271) Table centrifuge (Eppendorf, cat. no. 5430 R) Filter mesh, 100 μm (cut into 1.5 × 1.5–cm cubes and autoclaved; A. Hartenstein GmbH, cat. no. PES3) BD LSR Fortessa II

Techniques: Activity Assay, Lysis, Suspension, Transferring, Purification, Isolation, Cell Counting